# Unclear imaging

**URL:** <https://openuc2.discourse.group/t/unclear-imaging/46>\
**Category:** General\
**Tags:** imswitch\
**Created:** [June 24, 2024, 8:50am UTC](https://openuc2.discourse.group/t/unclear-imaging/46 "2024-06-24T08:50:09Z")\
**Posts on this page:** 5\
**Page:** 1

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**Author:** ![Yuhao](https://avatars.discourse-cdn.com/v4/letter/y/c68b51/32.png) [@Yuhao](https://openuc2.discourse.group/u/Yuhao)\
**Post date:** [June 24, 2024, 8:50am UTC](https://openuc2.discourse.group/t/unclear-imaging/46/1 "2024-06-24T08:50:09Z")

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Hi Benedict,

I am imaging the some Arabidopsis samples with a few leaves. They are unclear for me to recognize the roots

 ![Root image](https://global.discourse-cdn.com/free1/uploads/openuc2/original/1X/ec4cf15c00879f990815c829bb6502737c210cb2.png)

I am a beginner in microscopy. Could you tell me what parameters can be used to sharper the imaging?

Best  
Yuhao

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**Author:** ![benedictdied](https://avatars.discourse-cdn.com/v4/letter/b/96bed5/32.png) [@benedictdied](https://openuc2.discourse.group/u/benedictdied)\
**Post date:** [June 25, 2024, 6:50am UTC](https://openuc2.discourse.group/t/unclear-imaging/46/2 "2024-06-25T06:50:27Z")

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Hey @Yuhao, thanks for posting this image. Indeed, this looks really funky and blurry. Could you use a different sample first to test if there is any issue with the optics? Also, I made an introductory video here: [openUC2 FiveD v3 | openUC2 Documentation](https://openuc2.github.io/docs/Investigator/XYZMicroscope/FiveD_v3#introduction)  
Perhaps this helps a bit already? Can you also take a photo of the microscope eventually? Maybe something is misaligned. Was this fluorescence by the way?

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**Author:** ![benedictdied](https://avatars.discourse-cdn.com/v4/letter/b/96bed5/32.png) [@benedictdied](https://openuc2.discourse.group/u/benedictdied)\
**Post date:** [June 26, 2024, 7:10am UTC](https://openuc2.discourse.group/t/unclear-imaging/46/3 "2024-06-26T07:10:50Z")

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How is it going @Yuhao I hope the plants are imaged clearly now? 🙂

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**Author:** ![Yuhao](https://avatars.discourse-cdn.com/v4/letter/y/c68b51/32.png) [@Yuhao](https://openuc2.discourse.group/u/Yuhao)\
**Post date:** [June 27, 2024, 11:16am UTC](https://openuc2.discourse.group/t/unclear-imaging/46/4 "2024-06-27T11:16:57Z")

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Hi, @benedictdied! Thanks for your detailed reply. The issue is indeed partly caused by the terrible sample, and wrong setting of Imswitch. After replacing it with a better sample and adjusting the z-axis, I have a clear image now!

 ![IMG_9314.HEIC](https://global.discourse-cdn.com/free1/uploads/openuc2/original/1X/bdabba8c3ec9ad3be3244e53d7b97afe2b384b44.jpeg)

Cheers,  
Yuhao

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**Author:** ![benedictdied](https://avatars.discourse-cdn.com/v4/letter/b/96bed5/32.png) [@benedictdied](https://openuc2.discourse.group/u/benedictdied)\
**Post date:** [June 27, 2024, 11:34am UTC](https://openuc2.discourse.group/t/unclear-imaging/46/5 "2024-06-27T11:34:05Z")

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Beautifully horrifying 😉  
Thanks for sharing! 🙂